• Nader Srour, Olivier Lavoie, Axelle Khouma, Moein Minbashi Moeini, Julie Plamondon, Richard Kinkead, Natalie J Michael, and Alexandre Caron.
• Faculty of Pharmacy, Université Laval, Quebec City, QC, Canada; Quebec Heart and Lung Institute, Quebec City, QC, Canada.
• Neuroscience. 2023 Oct 15; 530: 9510795-107.

AbstractPro-opiomelanocortin (POMC)-expressing neurons in the arcuate nucleus of the hypothalamus (ARC) are considered a major site of leptin action. Due to increasing evidence that POMC neurons are highly heterogeneous and indications that the conventional molecular tools to study their functions have important limitations, a reassessment of leptin's effects on definitive POMC neurons is needed. POMC neurons are also expressed in the retrochiasmatic area (RCA), where their function is poorly understood. Furthermore, the response of POMC neurons to leptin in females is largely unknown. Therefore, the present study aimed to determine the differences in leptin responsiveness of POMC neurons in the ARC and the RCA using a mouse model allowing adult-inducible fluorescent labeling. We performed whole-cell patch clamp electrophysiology on 154 POMC neurons from male and female mice. We confirmed and extended the model by which leptin depolarizes POMC neurons, in both the ARC and the RCA. Furthermore, we characterized the electrophysiological properties of an underappreciated subpopulation representing ∼10% of hypothalamic POMC neurons that are inhibited by leptin. We also provide evidence that sex does not appear to be a major determinant of basal properties and leptin responsiveness of POMC neurons, but that females are overall less responsive to leptin compared to males.Copyright © 2023 IBRO. Published by Elsevier Ltd. All rights reserved.

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