• J H Chen, W Wu, H S Li, T Fagaly, L Zhou, J Y Wu, and Y Rao.
• Laboratory of Molecular Neurobiology, Shanghai Research Center for Life Sciences and The National Institute of Neuroscience, The Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai, China.
• Neuroscience. 2000 Jan 1; 96 (1): 231236231-6.

AbstractThe slit genes have recently been found to encode proteins with a conserved chemorepulsive activity for axons in invertebrates and vertebrates. We have determined the expression pattern of a slit gene in Xenopus embryos. In the neural tube, slit is expressed at the ventral and dorsal midlines, and the motor neurons. slit is also expressed in a changing pattern in the retina. The full-length Xenopus Slit protein is secreted extracellularly, whereas its receptor Roundabout can not be secreted. Using a myc-tagged secreted Slit protein, we confirmed the binding of Slit to Roundabout expressed on the cell surface. These results confirm Slit-Roundabout interactions and the biochemical properties of Slit and Roundabout proteins, and further support the idea that Slit may guide axon projections in multiple regions of the embryo.

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