• Methods Mol. Biol. · Jan 2012

    Induction of nitric oxide synthase-2 expression and measurement of nitric oxide production in enriched primary cortical astrocyte cultures.

    • James A Hewett and Sandra J Hewett.
    • Department of Biology, Syracuse University, Syracuse, NY, USA. jhewett@nso1.uchc.edu
    • Methods Mol. Biol. 2012 Jan 1; 814: 251-63.

    AbstractAstrocytes produce numerous mediators under conditions of inflammation in the central nervous system. One such mediator is nitric oxide (NO) derived from nitric oxide synthase-2 (NOS-2), the high output, inducible NOS isoform. Expression of NOS-2 and production of NO can be stimulated in astrocyte cultures by combinations of cytokines and lipopolysaccharide, a gram-negative bacterial endotoxin. This chapter details methods to induce and analyze NOS-2 expression and NO production in astrocyte cultures.

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