• Xiaofeng Xu, Zijing Liu, Hao Huang, Kang Zheng, Xuemei Hu, Zunyi Zhang, and Mengsheng Qiu.
• Institute of Developmental and Regenerative Biology, Zhejiang Key Laboratory of Organ Development and Regeneration, Hangzhou Normal University, Hangzhou, Zhejiang 310029, China; Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, KY 40292, USA.
• Neuroscience. 2016 Oct 28.

AbstractTo date, five AP-2 genes that encode AP-2α, β, γ, δ and ε have been identified in vertebrates and they have been reported to be key regulators of embryonic development. However, the role of AP-2 family members in the development of central nervous system (CNS) has not been characterized. In the present study, we systematically examined the spatiotemporal expression pattern of AP-2 genes in the developing spinal cord of mouse and chick embryos and found that AP-2α and AP-2β are specifically expressed in post-mitotic dorsal interneurons. Loss-of-function analysis using in ovo electroporation in embryonic chick spinal cord preliminarily demonstrated that cAP-2α and cAP-2β regulates dorsal Class A and Class B interneuron specification, respectively. Gain-of-function experiments further revealed that misexpression of cAP-2α, but not cAP-2β, was able to induce the ectopic generation of Class A interneurons. Together, our studies indicated that AP-2 family members, AP-2α and AP-2β, have distinct functions in the regulation of dorsal interneuron development.Copyright © 2016. Published by Elsevier Ltd.

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