Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals
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Comparative Study Observational Study
Neutrophil gelatinase-associated lipocalin after off pump versus on pump coronary artery surgery.
Cardiac surgery. ⋯ On-pump patients had greater neutrophil activation. On- versus off-pump surgery had similar impact on tubular cells.
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Establish the prognostic value for graft loss of urinary neutrophil gelatinase-associated lipocalin (uNGAL), kidney injury molecule-1 (uKIM-1), interleukin-18 (uIL-18), and heat shock protein 72 (uHsp72) in kidney transplant recipients (KTR) with acute kidney injury (AKI). ⋯ After 1 year, 11 KTR with graft loss had higher uNGAL compared to KTR without loss (p < 0.001). There were no differences for uKIM-1, uIL-18 and uHsp-72. uNGAL >200 ng/mL had 84% sensitivity and 86% specificity for graft loss (ROC AUC: 0.89, 95% CI: 0.81-0.97). uNGAL may be useful to predict graft loss after AKI.
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Neutrophil gelatinase-associated lipocalin (NGAL) is secreted by injured kidney cells as well as by activated neutrophils in response to bacterial infections. We assessed the influence of acute renal dysfunction on the association between plasma NGAL and sepsis. ⋯ Plasma NGAL can help clinicians to identify bacterial infections in critically ill patients.
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In this study, we describe the development and evaluation of a slide-based immunoassay platform for the detection of neutrophil gelatinase associated-lipocalin (NGAL) in plasma and urine samples. The capture NGAL antibody was immobilized onto a microscope slide before an analysis of NGAL based on a sandwich immunoassay was further carried out. ⋯ The coefficients of variability (CVs) indicated good reproducibility and repeatability of the system. The levels of plasma NGAL measured by the slide-based system were highly correlated with those of ELISA, while this system over-predicted urine NGAL.
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Randomized Controlled Trial
Urinary lipid and protein oxidation products upon halothane, isoflurane, or sevoflurane anesthesia in humans: potential biomarkers for a subclinical nephrotoxicity.
To investigate whether lipid and protein oxidation products are elevated and correlated with routine clinical markers of hepatic and renal function in patients anesthetized with halothane, isoflurane, or sevoflurane. ⋯ Lipid and protein oxidation contributes to subclinical sevoflurane nephrotoxicity. Oxidation products may serve as early biomarkers.