Bulletin of experimental biology and medicine
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Bull. Exp. Biol. Med. · Mar 2014
Neuroprotective effects of erythropoietin in focal brain ischemia in rats.
Neuroprotective effect of erythropoietin administered before ischemia has been previously demonstrated. The efficiency of erythropoietin administration after ischemia was not studied, though in case of success these protocols would be applied in clinical neurology. ⋯ Injection of erythropoietin in 3 and 12 h after ischemia significantly reduced the size of necrosis (p = 0.0007 and p=0.0016, respectively), neurological deficit (p=0.0013 and p=0.0062, respectively), and brain edema (p=0.02 and p=0.0186, respectively). Injection of erythropoietin after transitory focal cerebral ischemia produced a pronounced neuroprotective effect.
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Bull. Exp. Biol. Med. · Nov 2013
Effects of erythrocyte microvesicles on the coagulation process stages.
We studied the effect of erythrocyte microvesicles on various stages of platelet-free autoplasma coagulation process. The erythrocyte microvesicles not only exhibited procoagulant activity, but also produced a pronounced anticoagulant effect by inhibiting fibrin formation process. The effects of microvesicles isolated from erythrocytes after 24- and 48-hour cell storage on plasma coagulation were different. The hemocoagulation effects of the "light" and "heavy" microvesicles were studied.
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Bull. Exp. Biol. Med. · May 2013
Role of ryanodine receptors in the effects of hydrogen sulfide on transmitter release from the frog motor nerve ending.
We studied the role of ryanodine receptors in the effects of hydrogen sulfide on transmitter release from frog motor nerve ending. Sodium hydrosulfide (300 μM), a donor of hydrogen sulfide, reversibly increased the frequency of miniature endplate current without changes in its amplitude-time parameters. ⋯ Under conditions of ryanodine receptors blockade with ryanodine (10 μM), sodium hydrosulfide had no effect on induced transmitter release, but its effects remained unchanged during ryanodine receptors blockade with dantrolene (25 μM). We concluded that an enhanced acetylcholine release induced by hydrogen sulfide is related to an increase of intracellular Ca(2+)concentration due to activation of ryanodine receptors for intracellular Ca(2+)-pool.
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Bull. Exp. Biol. Med. · Dec 2012
Study of nociceptive flexion reflex in healthy subjects and patients with chronic neuropathic pain syndrome.
Nociceptive flexion reflex was measured in healthy subjects and patients with chronic neuropathic pain (diabetic distal symmetric sensorimotor polyneuropathy). The study of nociceptive flexion reflex revealed reduction of subjective pain threshold and reflex threshold in patients compared with healthy persons reflecting deficit of descending antinociceptive influences in the CNS.
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Bull. Exp. Biol. Med. · Aug 2012
Comparative StudyNeuroprotective effect of mesenchymal and neural stem and progenitor cells on sensorimotor recovery after brain injury.
We studied the effect of systemic administration of multipotent stem cells on impaired neurological status in rats with brain injury. It was found that transplantation of multipotent mesenchymal stromal cells of the bone marrow or human neural stem and progenitor cells to rats with local brain injury promoted recovery of the brain control over locomotor function and proprioceptive sensitivity of forelegs. ⋯ Transplantation of cell cultures improved survival of experimental animals. It should be noted that administration of neural stem and progenitor cells prevented animal death not only in the acute traumatic period, but also in delayed periods.